Fig. 2

Uev1A positively regulates expression of CT45A downstream genes in breast cancer cells. a, b Transcript levels of tumorigenic genes, including RASGEF1A, MAGED4B, HOXB6 and HOXD13 in CT45A-overexpressed MDA-MB-231 (a) and MCF7 (b) cells were detected by qRT-PCR. c, d Transcript levels of EMT, stemness and metastatic genes, including KIT, ALDH1A1, CXCR4, TWIST1 and/or SULF2 in CT45A-overexpressed MDA-MB-231 (c) and MCF7 (d) cells were detected by qRT-PCR. e, f Expression of CT45A downstream tumorigenic genes, including HOXB6, RASGEF1A, MAGED4B and/or HOXD13 in UEV1A transiently overexpressed MDA-MB-231 (e) and MCF7 (f) cells were monitored by qRT-PCR. g, h Expression of CT45A downsream EMT, stemness and metastasic genes, including ALDH1A1, KIT, CXCR4, SULF2 and/or TWIST1 were monitored in UEV1A transiently overexpressed MDA-MB-231 (g) and MCF7 (h) cells by qRT-PCR. CK, control treatment. All experiments were performed in at least triplicate and the results are the average with standard deviation. *, P < 0.05; **, P < 0.01; and ***, P < 0.001