Fig. 1

Uev1A upregulates CT45A expression in a Ubc13-dependent manner in breast cancer cells. a, b Relative CT45A transcript levels in UEV1A-overexpressed MDA-MB-231 (a) and MCF7 (b) cell lines were monitored by qRT-PCR. c, d MDA-MB-231 (c) and MCF7 (d) cells were transfected with shRNA lentiviral particles against UEV1A (shUEV1A) or non-specific target (shCK). shUEV1A-1 and shUEV1A-2 represent two independent stable shUEV1A cell lines. CT45A transcript levels in shCK and shUEV1A cell lines were monitored by qRT-PCR. e, f Overexpressed UEV1A but not UEV1A-F38E upregulated CT45A expression in MDA-MB-231 (e) and MCF7 (f) cells, as determined by qRT-PCR. CK, control treatment. All experiments were performed in at least triplicate and the results are the average with standard deviation. **, P < 0.01