Table 1 The primer sequences used in the current study
From: CML derived exosomes promote tumor favorable functional performance in T cells
Forward | Reverse | |
|---|---|---|
Gapdh | 5′-CCGAGCCACATCGCACAG-3′ | 5′-GGCAACAATATCCACTTTACCAG-3′ |
Β-actin | 5′-AGACGCAGGATGGCATGGG-3’ | 5′-GAGACCTTCAACACCCCAGCC-3’ |
TNF-α | 5′-ATGTTGTAGCAAACCCTCAAGC-3′ | 5′-AGGACCTGGGAGTAGATGAGG-3′ |
TGF-β | 5′- AGTGGACATCAACGGGTTCAC-3′ | 5′-TGGAGCTGAAGCAATAGTTGGTG-3′ |
IL-6 | 5′- GCACTGGCAGAAAACAACCT-3′ | 5′- CAGGGGTGGTTATTGCATCT-3′ |
IL-10 | 5′-TCTCCGAGATGCCTTCAGCAGA-3’ | 5′-TCAGACAAGGCTTGGCAACCCA-3’ |
IL-17 | 5′- CGGACTGTGATGGTCAACCTGA-3’ | 5′- GCACTTTGCCTCCCAGATCACA-3’ |
CD3d | 5′-AAGTGAGCCCCTTCAAGATACC-3’ | 5′-TCTGAGAGCAGTGTTCCCAC-3’ |
ITK | 5′-ACTCCTGAAGACAACAGGCGA-3′ ′ | 5′ATCCTTCATGCCCATTCCTGTC-3′ |
NFATC3 | 5′-GTGAAGCTCCTGGGCTATAACG-3′ | 5′-TATCTCTTGGCTTGCAGTAGCG-3′ |
NFATC4 | 5′-GAAGGGTGAGACGGACATCG-3′ | 5′-TTGGAGCCAGTCAGTACCAGT-3′ |
FOXO1 | 5′- GAGGGTTAGTGAGCAGGTTACAC-3’ | 5′- TGCTGCCAAGTCTGACGAAAG-3’ |
FOXP3 | 5′-TTCATCTGTGGCATCATCCGAC-3 | 5′- GTCGCATGTTGTGGAACTTGAAG-3′ |
PD-1 | 5′-CGTGGCCTATCCACTCCTCA-3’ | 5′-ATCCCTTGTCCCAGCCACTC-3’ |
NQO1 | 5′-CCTGCCATTCTGAAAGGCTGGT-3’ | 5′-GTGGTGATGGAAAGCACTGCCT-3’ |
C-myc | 5′-CCTGGTGCTCCATGAGGAGAC-3’ | 5′-CAGACTCTGACCTTTTGCCAGG-3’ |