Fig. 2
The establishment and evaluation of circulating miRNA detection methodology. a Extraction efficiency was compared between the miRNeasy Serum/Plasma Advanced Kit and TRIzol LS by RT-qPCR. b The standard curve of miR-1290 standards with different concentrations (100 nM, 10 nM, 1 nM, 10pM, 1pM, 0.1pM), y = −4.173x + 58.96, R2 = 0.9998. c Expression levels of circulating miR-16-5p were analyzed in breast cancer (n = 10) and rheumatoid arthritis (n = 10) by RT-qPCR. d Analysis of expression levels of endogenous and exogenous controls in PC, GC and control group by RT-qPCR. d Expression levels of miR-1290 were assessed in the serum and plasma of the same individuals by RT-qPCR, with miR-16-5p as an endogenous control. e Expression levels of miR-1290 were assessed in the serum and plasma of the same individuals by RT-qPCR, with cel-miR-39 as an exogenous control. * p < 0.05, *** p < 0.001, ns: no significance