Fig. 6

Re-localization of fluorescence labeled Taxol to lysosomes following ultrasound. A Breast and ovarian cancer cells were added with fluorescence labeled Taxol analog (488-PTX) (1 nM) and Lyso-marker for 60 min. The cells on tissue culture dish were exposed to ultrasound (+ US, 40 KHz, 1.0 W/cm²) for 5 min. Representative 488-PTX images were taken before (control) and after exposure to ultrasound. The signals from 488-PTX and Lyso-Marker were combined/overlaid. An example of A2780 cells is shown. B Cells incubated with fluorescence labeled Taxol analog (488-PTX) (1 nM), ER-Tracker™, and Lysomarker (BioTracker LYSO-TP Live Cell Dye) markers for 60 min before exposure to ultrasound for 5 min. After exposure to ultrasound at time 0 (no ultrasound), 5, 10, 30 min, the cells were imaged. Representative images of OVCAR5 cells are shown