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Fig. 1 | BMC Cancer

Fig. 1

From: Long non-coding RNAs HERH-1 and HERH-4 facilitate cyclin A2 expression and accelerate cell cycle progression in advanced hepatocellular carcinoma

Fig. 1

lncRNAs HERH-1/4 exhibited higher levels in radvanced HCC cells. a Cluster analysis of the lncRNA and mRNA profiles based on microarray data from two pairs of HCC recurrent (RE) and primary (PR) tissues in order to evaluate the significance of RNA profile on different tissue types. b, c The level of the two selected lncRNAs, HERH-1 and HERH-4, were examined in additional ten pairs of HCC recurrent (RE) and primary (PR) tissues by qRT-PCR to validate their level alteration. d In order to know the lncRNA level in HCC cell lines, the relative HERH-1/4 level in an immortalized benign hepatocyte cell line HL-7702 and four HCC cell lines were detected by qRT-PCR (n = 2). The lncRNA level in HL-7702 cells was set to 1. e The existence and general length of HERH-1/4 in HCC cells were detected by Northern blot. f, g The subcellular location of HERH-1/4 in HCC cells were detected by FISH (f) and qRT-PCR (g, n = 2) assays. Scale bar, 50 μm. h The gene signature of the HCC recurrent tissues was analyzed using the gene set enrichment analysis (GSEA) database. The three enrichment plots show the similarity of the recurrent HCC gene profile with the three RB-associated gene sets. *P < 0.05

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