Fig. 7
From: Alectinib treatment improves photodynamic therapy in cancer cell lines of different origin
Apoptosis Induction by Alectinib and light treatment upon caspase inhibition. LED Light exposure enhances Alectinib-induced cell death in the cancer cell lines MDA-MB-231, MKN-28 and OE33 also in the absence of ALA. In all four cell lines (including SW-480) cell death induced by PDT after Alectinib treatment is further stimulated by 5-ALA. In order to investigate the role of caspases for Alectinib/PDT on a functional level, caspase activity was blocked by incubation of the cells with 10 μM of the cell-permeable, broad-spectrum caspase inhibitor Q-VD-Oph 2 h prior Alectinib/ALA/Light treatment. FACS analysis of treated cells revealed that caspase inhibition potently reduced the proportion of apoptotic cells in all cell lines. (Data show mean ± SD of three independent experiments; * p < 0.05)