Fig. 5

Apoptosis induction by Alectinib and light treatment in carcinoma cell lines. A) Cells of various cancer cell lines such as the breast carcinoma line MDA-MB-231, esophageal adenocarcinoma OE33, gastric carcinoma MKN28, colorectal carcinoma SW480 and NSCLC cells H460, H1299 were treated with DMSO alone or with 10 μM Alectinib/DMSO, 150 μM ALA, and LED light. Cells were stained using an Annexin V-FITC detection kit, counterstained by PI and apoptotic cells were determined by flow cytometry. Annexin V-positive/PI-negative cells were considered as early apoptotic cells, Annexin V-positive/PI-positive cells were late apoptotic/necrotic cells. Combined treatment of cells with Alectinib, ALA and LED light led to enhanced cell death in all the cell lines. (mean ± SD of three independent experiments; * p < 0.05) B) Densitometric analysis of Western Blot reveals that all cell lines strongly express FECH and that FECH expression levels were not changed by Alectinib treatment, except for SW480 cells where expression is slightly reduced. Level of FECH expression was normalized to respective actin control and presented as relative protein expression level in relation to respective wild type control. (Analysis was performed twice. Both WB including quantification and indication of the mean value are shown in the supplemental Fig. 2)