Fig. 2

Addition of ALA increased Alectinib-stimulated PpIX-fluorescence breast cancer cells. Human breast cancer cell lines MDA-MB-231 were incubated with 10 μM Alectinib for 24 h. Subsequently, cells were treated with ALA (X-axis) for 3 h. PpIX-fluorescence (Y-axis, arbitrary units) of the cells was directly assessed by flow cytometry with an excitation of 405 nm and emission at 698 nm (Filter 695/40). Treatment with Alectinib (dashed line) dissolved in DMSO resulted in a higher PpIX-fluorescence compared to treatment with DMSO alone (solid line) for concentrations of ALA up to 250 μM. Depicted is the median of three measurements