Fig. 7From: Interaction between DNMT3B and MYH11 via hypermethylation regulates gastric cancer progressionDNMT3B upregulation is responsible for the hypermethylation of the MYH11 promoter. A, the expression of DNMT3A and DNMT3B in GC was queried in StarBase Pan-cancer platform; B, the enrichment ability of DNMT3A and DNMT3B on MYH11 promoter examined by ChIP-qPCR; C, DNMT3B mRNA expression in GC tumor tissues and their adjacent tissues by RT-qPCR; D, correlation of DNMT3B expression with MYH11 promoter methylation levels in tumor tissues analyzed by Pearson’s correlation analysis (r = 0.623, p < 0.001); E, correlation of DNMT3B expression with MYH11 expression in tumor tissues (r = − 0.609, p < 0.001); F, transfection efficiency of oe-DNMT3B in GC cells by RT-qPCR; G, the effects of DNMT3B overexpression on the methylation level of MYH11 promoter examined by qMSP; H, effects of DNMT3B overexpression on MYH11 expression by RT-qPCR. Each assay was performed at least three times. Statistical significance was analyzed by paired t test (panel C) or two-way ANOVA (panels B, F, G and H) and Tukey’s multiple range testsBack to article page