Fig. 6

MYH11 promoter is methylated in GC. A, the MYH11 promoter CpG island was queried in NCBI; B, validation of CpG island sequences; C, the methylation level of MYH11 promoter in GC tumor tissues and their adjacent tissues examined by qMSP; D, the methylation level of MYH11 promoter in GSE-1 cells and GC cells examined by qMSP; E, the effect of 5-aZa-CDR treatment on the methylation level of MYH11 promoter in GC cells examined by qMSP; F, the effects of 5-aZa-CDR treatment on MYH11 expression in GC cells by RT-qPCR. Each assay was performed at least three times. Statistical significance was analyzed by paired t test (panel C), one-way (panel D) or two-way ANOVA (panels E and F) and Tukey’s multiple range tests