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Fig. 1 | BMC Cancer

Fig. 1

From: Inhibition of STAT3 enhances sensitivity to tamoxifen in tamoxifen-resistant breast cancer cells

Fig. 1

TamR cells have G1/S cell cycle progression and EGFR overexpression. a MCF-7 and TamR cells were treated with each concentrations of tamoxifen (4-OH-TAM) for 72 h and cell viability was measured using a CCK-8 kit. b Flow cytometric analysis of the cell cycle was performed in MCF-7 and TamR cells. TamR cells showed the increased G1 to S phase transition. c The protein expression levels of ERα and EGFR were assessed by immunoblotting in MCF-7 and TamR cells. GAPDH was used as the loading control. The cropped blots are used in the figure. The membranes were cut prior to exposure so that only the portion of gel containing the desired bands would be visualized. Data are expressed as mean ± SD, and all experiments were performed in triplicate, independently. P values calculated using an unpaired t-test. *p < 0.05, **p < 0.01, ***p < 0.001

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