Fig. 5

PRRX1 and metabolism of HCC cells. A Glucose consumption and lactate output were measured 48 h after siPRRX1 transfection. Bars indicate mean ± SD and a representative picture of 3 experiments is shown, each in triplicate. B Expression of genes involved in glycolysis were determined by qPCR 48 h after siPRRX1 transfection in HUH7 and HLF cells. Bars indicate mean ± SD and representative of 3 experiments each in triplicates. C Expression of genes involved in the TCA cycle were analyzed by qPCR 48 h after siPRRX1 transfection in HUH7 and HLF cells. Bars indicate mean ± SD and representative of 3 experiments, each in triplicates. D Expression of genes involved in amino acid metabolism as determined by qPCR 48 h after siPRRX1 transfection in HUH7 and HLF cells. Bars indicate mean ± SD and representative of 3 experiments each in triplicates. E Alterations of metabolites. Heatmap of changes in metabolite levels of glycolysis, TCA cycle and amino acids in HUH7 and HLF cells 48 h after siPRRX1 transfection. n = 3 per cell line and condition. White numbers indicate a p < 0.05; black numbers are not significant. The experiment was performed in triplicate