Fig. 3

ZEB1 and ZEB2 are novel PRRX1 related genes. A Volcano plot showing genes differentially expressed in PRRX1-high tumours from TCGA dataset. B Kaplan Meier OS analysis (logrank test) of high PRRX1 combined with low ZEB1 or high ZEB2 in GSE14520 dataset, based on its negative correlation with ZEB1 and positive correlation with ZEB2 in this dataset. H = high, L = low. C Pearson correlation of PRRX1 and ZEB1 or ZEB2 in two HCC collectives. D ZEB1 and ZEB2 expression in two representative HCC cohorts. Data was analysed with Student’s t-test. NT – non-tumour. TCGA: NT n = 50, HCC n = 373; GSE14520: NT n = 220, HCC n = 225. (E) Differential expression of ZEB1 and ZEB2 compared to PRRX1 in HCC cell lines. Cells were cultured for 48 h prior to analysis, bars indicate mean ± SD, n = 3 per group