Fig. 1

PRRX1 is upregulated in human HCC and shows a variable pattern in vitro. A PRRX1 expression in human HCC cohorts. NT - non-tumour, HCC - hepatocellular carcinoma. Data was analysed with Student’s t-test. Information about sample size per cohort is contained in Table S1. B PRRX1 expression in tumours showing its gene amplification. Data was analysed with Student’s t-test. Amp: amplification C PRRX1 expression in tumours with TP53 and CTNNB1 mutation in TCGA liver cancer cohort. Data was analysed with Student’s t-test. D Kaplan-Meier overall survival analysis (logrank test) based on PRRX1 expression in GSE14520 dataset. High PRRX1 n = 110; low PRRX1 n = 110. E PRRX1 expression in human HCC cell lines after 48 h culture. Normalized to the basal expression in HLF (lowest PRRX1 level). Bars indicate mean ± SD, n = 3 per group. F Ratio of PRRX1a to PRRX1b isoform expression in HCC cell lines after 48 h culture. Bars indicate mean ± SD, n = 3 per group