Fig. 2

HECW1 expression is negatively associated with tumor stage, bone metastasis, and targeted drug resistance in ccRCC. (a) Real-time PCR was used to detect the mRNA expression of HECW1 in HK-2, 786-O or 769-P, and Caki-1 cells. (b) Representative images of H&E and IHC staining for HECW1 in ccRCC tissues with different tumor node metastasis (TNM) stages are shown (scale bar = 20 μm). The expression of HECW1 in ccRCC specimens was evaluated by the H-score method, and the values are represented as the mean ± SD. (c) Representative images of H&E and IHC staining for HECW1 in tissues of localized ccRCC and ccRCC with bone metastasis are shown (scale bar = 20 μm). The expression of HECW1 in ccRCC specimens was evaluated by the H-score method, and values are represented as the mean ± SD. (d-e) Real-time PCR was employed to detect the mRNA expression of HECW1 in 786-O-SR (d) and 786-O-PR (e) cells. (f-g) Representative images of H&E and IHC staining for HECW1 in sunitinib-resistant (f) or pazopanib-resistant (g) orthotopic ccRCC specimens are presented (scale bar = 20 μm). Values are represented as the mean ± SD. All p values are defined as ***p < 0.001