Fig. 4

NR1D1 inhibited the growth of ovarian cancer cells in vivo. Ovarian cancer cells with stable transfection of NR1D1 over-expression plasmid were subcutaneously injected into the left flank of nude mice. Twenty-one days later, the tumors were obtained. A The images of tumors and tumor weight. B HE and TUNEL assay was performed to determine the histopathological changes and apoptosis in tumors. Typical images were presented. Scale bar = 50 μm. Red arrows indicated TUNEL positive cells. TUNEL-positive cells were counted. C The level of Ki-67 in tumors was determined by immunofluorescence. Scale bar = 50 μm. White arrows indicated Ki-67 positive cells. The fluorescence intensity of Ki-67 was examined. D The levels of phosphorylated JAK1, phosphorylated JAK2 and phosphorylated STAT3 in tumors with NR1D1 over-expression were determined with western blot