Fig. 3
From: Correction to: Inhibition of mTORC1 through ATF4-induced REDD1 and Sestrin2 expression by Metformin
Metformin induces the expression of REDD1 and Sestrin2 in an ATF4-dependent manner. (a, b, e, f) H1299 cells were treated with the indicated concentrations of metformin or phenformin for 24 h. (c, d) H1299 cells were transfected with control or ATF4 siRNA for 12 h and were then treated with 10 mM metformin for 24 h. The protein levels (a, c, e) and mRNA levels (b, d, f) were estimated by western blot and real-time PCR analysis, respectively. (a, c, e) The western blot is representative of two independent experiments. (b, d, f) The real-time PCR results for each sample were analysed according to the 2−ΔΔCt method using β-actin as the internal control. Gene transcription is presented as the fold change relative to the control sample (n = 3; ***P < 0.001). CTL: control, SESN1: Sestrin1, SESN2: Sestrin2, SESN3: Sestrin3