Fig. 2
From: Correction to: Inhibition of mTORC1 through ATF4-induced REDD1 and Sestrin2 expression by Metformin
Metformin inhibits mTORC1 through ATF4. (a, b) H1299 cells were treated with the indicated concentrations of metformin for 24 h. (c, d) H1299 cells were treated with 10 mM metformin for the indicated time. The protein levels (a, c) and mRNA levels (b, d) were estimated by western blot and RT- PCR analysis, respectively. (a-d) Data are representative of two independent experiments. (e, f) H1299 cells were transfected with control or ATF4 siRNA for 12 h and were then treated with 10 mM metformin for 24 h. (e) Data are representative of three independent experiments. (f) The p-S6K expression was quantified using ImageJ software and fold change with respect to control after normalization to respective S6K bands was plotted as histogram. (n = 3; ***P < 0.001; NS, not significant). CTL: control