Fig. 2

Rhabdoid tumor is more sensitive to in vitro inhibition of growth factor receptors than epithelioid sarcoma. A Half-maximal inhibitory concentration (IC₅₀) after 72 h, based on confluence (IncuCyte®) or MTS assay (*) in N ≥ 3 biological replicates. B EGFR, FGFR1 and FGFR2 mRNA expression levels of A204, VAESBJ, and GRU1 cells were determined by RT-qPCR. The scatter plot shows measurements of N ≥ 3 biological replicates in 3 technical replicates, normalized on GAPDH expression (dCT (cycle threshold) values; median ± IQR ). C Cell proliferation in response to EGF and FBS10% in serum-starved cells by IncuCyte®, graph represents means ± SEM of N ≥ 3 biological replicates. D Effects on AKT and ERK activity after treatment with pazopanib (left panel) and regorafenib (right panel) after 5, 30 and 120 min, assessed by Western blot (ratio candidate protein/β-ACTIN, related to expression of control)