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Fig. 2 | BMC Cancer

Fig. 2

From: TRIM27 interacts with Iκbα to promote the growth of human renal cancer cells through regulating the NF-κB pathway

Fig. 2

TRIM27 silencing suppressed the proliferation and promoted apoptosis of human renal cells. A & B. TRIM27 siRNAs deeply suppressed the relative mRNA and protein content of TRIM27 in human Caki-2 and 786–0 cells, respectively. ***p < 0.001 vs siNC. The mRNA levels of TRIM27 was used 2−ΔΔCt method and normalized to that of GAPDH. C & D. siTRIM27–1 and siTRIM27–2 significantly suppressed the proliferation of Caki-2 and 786–0 cells. *p < 0.05 vs siNC, ***p < 0.001 vs siNC. E. Apoptosis of Caki-2 and 786–0 cells was significantly increased following transfecting with siTRIM27–1 and siTRIM27–2 at 48 h, respectively. ***p < 0.001 vs siNC. F & G. Western blot was used to examine the protein contents of TRIM27, cleaved caspase-3, NF-κB (cytoplasm), NF-κB (nuclear) and IkBa in Caki-2 cells (F) and 786–0 cells (G) transfected with siNC, siTRIM27–1, or siTRIM27–2 at 48 h respectively. *p < 0.05 vs siNC, **p < 0.01 vs siNC, ***p < 0.001 vs siNC

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