Fig. 5

Aneuploid pleomorphic sarcoma cells are sensitive to AICAR treatment. A Growth of IB105 DsRed, IB106 GFP and IB105/106 hybrids in absence or presence of AICAR treatment (700 μM for 6 days) evaluated by flow cytometry. For each cell line, data are expressed as growth percentage compared to the growth of the untreated sample (100%). Statistical tests were done using a t-test (nonparametric) Mann-Whitney (**p < 0.01, error bars, SD; n = 5 done in sextuplicates twice and in triplicates for the rest of the experiments). B Migration of IB105 DsRed, IB106 GFP and IB105/106 cells post-vehicle or AICAR pre-treatment (700 μM for 24 h) evaluated by wound healing assays using IncucyteS3. Data represent relative wound density at 8 or 24 h post-scratch. Statistics were done using a t-test. (Nonparametric) Mann-Whitney (***p < 0.001, ****p < 0.0001, error bars, SD; n = 2 done in 12 wells for each sample, condition and per experiment)