Fig. 6

ALX3 recruits KDM2B to the promoter region of CDC25A to strengthen the CDC25A transcription through demethylation of H3K4me3. A methylation modification of the CDC25A histone promoter in cervical cancer cells predicted on UCSC browser; B expression of H3K4me3 in CSCC cells (C33A, Caski, HeLa and ME180) and in PCS480 cells determined using western blot analysis; C correlations between CDC25A and KDM1A, KDM2B, KDM5A/B/C/D predicted on the TCGA-CESC database; D-E, mRNA (D) and protein (E) expression of KDM2B in CSCC cells and in PCS480 cells determined by RT-qPCR and western blot analysis, respectively; F H3K4me3 expression in CDC25A promoter region in CSCC cells and in PCS480 cells determined by the ChIP-qPCR assays; G subcellular localization of ALX3 and KDM2B in ME180 and C33A cells examined by double-labeled immunofluorescence staining; H binding relationship between KDM2B and ALX3 in ME180 and C33A cells examined by the Co-IP assay. Data were presented as the mean ± SD from three independent experiments. Data were analyzed by two-way ANOVA followed by Tukey’s multiple comparison test. In panels B, D, E and F, ***p < 0.01 vs. PCS480. All cellular experiments were performed in triplicates and three duplicated wells were set