Fig. 5

Overexpression of ALX3 blocks the inhibition of sh-CDC25A on CSCC cells. A-B transfection efficacy of oe-ALX3 in ME180 and C33A cells determined by RT-qPCR (A) and western blot analysis (B); C-D cell cycle progression (C) and apoptosis (D) of ME180 and C33A cells determined by flow cytometry; E number of apoptotic cells determined by TUNEL assay; F-G proliferation ability of cells determined by colony formation (F) and EdU labeling (G) assays. Data were presented as the mean ± SD from three independent experiments. Data were analyzed by two-way ANOVA followed by Tukey’s multiple comparison test. **p < 0.01 vs. shCDC25A-1 + oe-NC. All cellular experiments were performed in triplicates and three duplicated wells were set