Fig. 2

Knockdown of CDC25A suppresses proliferation but promotes cell cycle arrest and apoptosis of CSCC cells. A-B two shRNAs were administrated in ME180 and C33A cells, after which the CDC25A expression in cells was determined by RT-qPCR and western blot analysis, respectively; C-D cell cycle progression and apoptosis of ME180 and C33A cells determine by flow cytometry; E phosphorylation of CDK2 in ME180 and C33A cells examined by western blot analysis; F number of apoptotic cells determined by TUNEL; G-H proliferation of cells determined by colony formation and EdU labeling assays. Data were presented as the mean ± SD from three independent experiments. Data were analyzed by two-way ANOVA followed by Tukey’s multiple comparison test. In all panels, **p < 0.01 vs. sh-NC. All cellular experiments were performed in triplicates and three duplicated wells were set