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Fig. 4 | BMC Cancer

Fig. 4

From: Formin-like protein 2 promotes cell proliferation by a p27-related mechanism in human breast cancer cells

Fig. 4

Effects of FMNL2 overexpression on cell proliferation in MCF7 cells. After transfection for 48 h, cultured cells were processed for indicated assays. a The levels of FMNL2, p27, p21, CyclinD1, and β-actin proteins were determined by western blotting and representative blots were shown. b The cell viability was examined by MTT assay. c The number of forming colonies was counted and analyzed statistically. d Representative percentage of cells in the G0/G1, G2/M and S phases was detected by flow cytometric analysis. e The CDK4/CyclinD1 kinase activity was determined. *: P < 0.05 vs NC vector group

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