Fig. 5

MAP3K13 is a target of miR-4500 and is indirectly regulated by LINC01287. A, heat map showed the top 40 upregulated mRNAs in 4 colon cancer tissues and paired normal samples identified by transcriptome sequencing. B, spearman analysis of correlation between MAP3K13 and miR-4500 expression in 60 colon cancer patients. C, the predicted binding sites of MAP3K13 and miR-4500. D, The pMIRREPORT plasmid containing the wt or mt 3′ UTR of MAP3K13, the miR-4500 expression plasmid or miR-ctrl, and a renilla luciferase plasmid (2: 2: 1) were transient transfected into HEK293T cells for luciferase reporter assay. E, the levels of MAP3K13 pulled down by biotinylated wt or mt miR-4500, or non-targeting control (NC) were tested by qRT-PCR. F, SW480 and HCT116 cells were infected with LINC01287, EV, miR-4500 or miR-ctrl lentiviral particles as indicated, then protein expression of MAP3K13 was tested by western blot. Uncropped gels were in Supplementary Fig. 1. G, SW480 and HCT116 cells were infected with sh-LINC01287–1, sh-LINC01287–2 or sh-ctrl lentiviral particles, then protein levels of indicated genes were tested by western blot. Uncropped gels were in Supplementary Fig. 2. H, spearman analysis of correlation between LINC01287 and MAP3K13 expression in 60 colon cancer patients. Compared with wt MAP3K13 or Bio-NC group using two-tailed Student’s t-test, *p ≤ 0.05, **p ≤ 0.001. n.s. = not significant