Fig. 6

Migration and anchorage-independent growth assessment of the DCIS cell lines. Migratory capacity of the DCIS cell lines, in comparison to MCF10, MCF7 and MDA-MB-231 cells, was examined by a wound healing assay. The wound was imaged using bright field microscopy at 20X (A) and pseudo-marked in black. B wound width was quantified in ImageJ in arbitrary units (N = 3). Anchorage-independent growth was measured by soft agar assays; wells were imaged using the Odyssey Li-Cor system (D) and scored manually (E). Shown in panel E are two biological replicates (black/blue), with each technical replicate plotted. Horizontal bars show the mean number of colonies formed +/− SEM (GraphPad Prism v.8.3.0)