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Fig. 3 | BMC Cancer

Fig. 3

From: Molecular and cellular characterization of two patient-derived ductal carcinoma in situ (DCIS) cell lines, ETCC-006 and ETCC-010

Fig. 3

Molecular subtype based on hormone-receptor status of DCIS cell lines, ETCC-006 and ETCC-010. Estrogen receptor alpha (ERα), progesterone receptor (PR) and receptor tyrosine kinase ErbB-2 (HER2) levels were measured at the protein level (A) and (B) and RNA level (C) by immunoblotting and qRT-PCR. For the immunoblot, 42 μg of total protein was loaded per lane onto the gel. Protein level was quantified and normalized against βactin using ImageJ. For quantification in panel B, the horizontal bar indicates the mean with +/−standard error of mean (SEM). This experiment was repeated in triplicate and shown is a representative immunoblot. Full-length blots are presented in Supplementary Materials

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