Table 2 Sequence of primers and siRNA used in the study
From: Pyruvate carboxylase promotes thyroid cancer aggressiveness through fatty acid synthesis
Gene | Prime (5′ → 3′) |
|---|---|
Primers used in RT-PCR analysis | |
 PC | Forward: ATGTTGCCCACAACTTCAGCAAGC |
Reverse: AGTTGAGGGAGTCAAACACACGGA | |
 SREBP1c | Forward: GGAGCCATGGATTGCACATT |
Reverse: CAGGAAGGCTTCCAGAGAGG | |
 FASN | Forward: GGTCTTGAGAGATGGCTTGC |
Reverse: AATTGGCAAAGCCGTAGTTG | |
 ACC | Forward: GATCAAACTCTGGGAGTCTATG |
Reverse: TCTCGGCCTTCTGGATATT | |
 ACLY | Forward: CAACTTTGCCTCTCTCCGCTCTG |
Reverse: TCCCTTCTGGTCCGCCTTCTTG | |
 β-actin | Forward: GCACCACACCTTCTACAATG |
Reverse: TGCTTGCTGATCCACATCTG | |
Primers used in siRNAs | |
 siCtrl | Forward: UUCUCCGAACGUGUCACGUTT |
Reverse: ACGUGACACGUUCGGAGAATT | |
 siSREBP1c | Forward: GCAAGGCCAUCGACUACAUTT |
Reverse: AUGUAGUCGAUGGCCUUGCTT | |
Primers used in gene cloning | |
 SREBP1c | Forward: TCCGGTGAATTCCTCGAGCCACCATGGATTGCACTTTCGAAGAC |
Reverse: TCGCAGATCCTTGGATCCCTAGCTGGAAGTGACAGTGGTC | |
 PC | Forward: GATCCGCCAGACAACGTGGTCTTCAAGTTCTTTCAAGAGAA GAACTTGAAGACCACGTTGTCTGGTTTTTTC |
Reverse: AATTGAAAAAACCAGACAACGTGGTCTTCAAGTTCTTCTCTT GAAAGAACTTGAAGACCACGTTGTCTGGCG | |