Fig. 7
From: Pyruvate carboxylase promotes thyroid cancer aggressiveness through fatty acid synthesis
PC enhances the aggressiveness of TC cells by reprogramming fatty acid metabolism. A Western blot analysis of PC and SREBP1c protein expression in different 8505C cells (PC-WT, PC-sh, and PC-sh + SREBP1c-OE); the full blot is provided in the supplementary file. B The CCK-8 assay was used to detect the proliferation of the indicated 8505C cells. C Transwell invasion and metastasis assays were applied to assess the motility of different 8505C cells with a 40× objective lens. * p < 0.05; *** p < 0.01. The number of invaded cells is shown in histograms on the right. D Tumor growth was monitored in the indicated 8505C cells, and explanted tumor images from sacrificed mice are shown. * p < 0.05; *** p < 0.01. The number of invaded cells is shown in histograms on the right. Note: OE (overexpression), CCK-8 (Cell Counting Kit-8)