Fig. 3
From: Pyruvate carboxylase promotes thyroid cancer aggressiveness through fatty acid synthesis
Upregulation of the expression of lipogenic enzymes by PC in TC cells. A qRT-PCR analysis of mRNA expression and the lipogenic enzyme genes FASN, ACC1, and ACLY in 8505C and TPC1 cells. B Western blot analysis of the protein level of the lipogenic enzyme FASN in the indicated cells, the full blot is provided in supplementary file. C Results from the correlation analysis between mRNA expression levels of PC and FASN in 38 TC tissues, as show in the scatter plot. D IHC staining was used to analyze the relationship between the protein expression levels of PC and FASN. E Western blot analysis of the protein level of the lipogenic enzymes ACC1 and ACLY in the indicated cells; the full blot is provided in the supplementary file. Note: FASN (fatty acid synthesis), ACC1 (acetyl-CoA carboxylase), ACLY (ATP-citrate lyase), IHC (immunohistochemistry), qRT-PCR (quantitative reverse transcription PCR)