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Fig. 5 | BMC Cancer

Fig. 5

From: Development of a liquid biopsy based purely quantitative digital droplet PCR assay for detection of MLH1 promoter methylation in colorectal cancer patients

Fig. 5

Determining an optimal cut-off to distinguish healthy from CRC patients. a. Based on ddPCR LOD at 10% methylation (approximately 8 positive droplets), all 20 normal patient samples fell below the limit of detection and 21/27 (77.8%) of cancer patients were above this threshold. b. A tiered-based cutoff (20th, 50th, 80th percentile based) to distinguish “methylation high” CRC samples from “methylation low” CRC samples

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BMC Cancer

ISSN: 1471-2407

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