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Fig. 3 | BMC Cancer

Fig. 3

From: Development of a liquid biopsy based purely quantitative digital droplet PCR assay for detection of MLH1 promoter methylation in colorectal cancer patients

Fig. 3

MLH1 methylation detection of spike in normal plasma sample. Standard curve generated by spiking in 50 ng of DNA with known percentage of methylation into normal plasma, extracting cfDNA from the sample and testing in ddPCR. The normal samples had a measured baseline level of 0 positive droplets. The standard curve gave a R2 value of 0.985 and the equation of best can be used to relate positive droplet count to percent methylation

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BMC Cancer

ISSN: 1471-2407

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