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Fig. 2 | BMC Cancer

Fig. 2

From: Development of a liquid biopsy based purely quantitative digital droplet PCR assay for detection of MLH1 promoter methylation in colorectal cancer patients

Fig. 2

Sample input optimization for MLH1 promoter methylation ddPCR assay. With 0.125-4 ng input of synthetic DNA, MLH1 methylation levels detected in the methylated treated, methylated untreated, and unmethylated untreated sample remained fairly constant, the unmethylated enzyme treated sample showed a significant reduction in signal (P < 0.001). To achieve the optimal signal/noise ratio and operational/practical advantage, 1 ng was selected as the optimal input for further analysis

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