Fig. 1

Titration of input amount using synthetic DNA to determine minimum input for qPCR and ddPCR. a. Linearity of MLH1 qPCR assay showed very good linearity 0.9987 and efficiency 94.98% with an input range of 100 ng–3.125 ng control DNA. b. Comparative analysis of MLH1 promotor methylation using ddPCR demonstrated the ability to detect MLH1 copies in this assay at very low input level (< 1 ng equivalent input). As low as 0.096 ng of MLH1 methylation DNA could be detected by ddPCR