Fig. 6

NFATc2 regulated ROS metabolism involved in OPNc-CM promoted cell survival of A549 cells with cisplatin treatment. a Apoptosis analyses by flow cytometry in A549 cells treated with cisplatin (20 μg/ml) for 48 h in OPNc-CM and CM with OPN-neutralizing antibody. b Quantification of apoptosis from conditions as in a, n = 3. c Western blot analyses for cytoplasmic or nuclear distribution of NFATc2 in A549 cells treated with OPN-CMs. d ROS cytometry in the NFATc2 or mutants transfected A549 cells treated with 10 μg/ml cisplatin in OPNc-CM for 12 h. Data for quantification were represented as the mean ± SD from 3 (for apoptosis and ROS detections) independent experiments. * p < 0.05, ** p < 0.01