Fig. 3

Nuclear accumulation of NFATc2 in cells treated with OPN-CMs. a Immunofluorescent images for NFATc2 subcellular localization in cells induced by OPNc-CM and CM with addition of OPN-neutralizing antibody or FK506 as an inhibitor to calcineurin. Twenty individual cells were selected for quantitative analyses of the ratio between nuclear localization and cytoplasmic localization for each NFATc2 as shown in the plot. b Quantification of NFATc1-c4 nuclear localization from immunofluorescence in cells treated with OPN-CMs for 3 h. c Western blot analyses for cytoplasm or nuclear distribution of NFATc2 and NFATc3 proteins in cells treated with OPN-CMs, where tubulin and coilin were used as cytoplasm and nuclear makers respectively. d Western blot analyses for cytoplasm or nuclear distribution of NFATc2 and NFATc3 proteins in cells treated with OPNc-CM and CM with addition of OPN-neutralizing antibody or FK506. * p < 0.05, ** p < 0.01, *** p < 0.001