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Fig. 2 | BMC Cancer

Fig. 2

From: Osteopontin isoform c promotes the survival of cisplatin-treated NSCLC cells involving NFATc2-mediated suppression on calcium-induced ROS levels

Fig. 2

Addition of conditioned medium from OPNc overexpressed cells increased intracellular calcium levels in H1299 cultures. a Live fluorescence images for [Ca2+]i in H1299 cells transfected with GCaMP6s-tdTomato plasmid and stimulated with OPNc-CM, CM with addition of OPN-neutralizing antibody and integrin αvβ3-blocking antibody (ab78289). b Quantitative analyses for [Ca2+]i in H1299 cells transfected with GCaMP6s-tdTomato and stimulated with OPN-CMs. c Quantitative analyses for [Ca2+]i in H1299 cells stimulated with OPNc-CM, CM with addition of OPN-neutralizing antibody and integrin αvβ3-blocking antibody. A total of 10 to 30 individual cells were analyzed for ratios of GCaMP6s over tdTomato fluorescence intensities. Data were represented as the mean ± SD

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