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Fig. 1 | BMC Cancer

Fig. 1

From: Osteopontin isoform c promotes the survival of cisplatin-treated NSCLC cells involving NFATc2-mediated suppression on calcium-induced ROS levels

Fig. 1

Decreased sensitivity to cisplatin observed in OPNc conditioned cultures of NSCLC cells. a Survival of H1299 cells cultured in OPN-SI CMs in the dose-response of cisplatin by CCK8 assays. b Cell apoptosis quantified from H1299 cells cultured in OPN-CMs and treated with cisplatin 30 μg/ml for 48 h. c Effect of OPNc on apoptosis as shown from the sample results of flow cytometry. d Quantification of cell apoptosis in H1299 cells treated with cisplatin 30 μg/ml for 48 h cultured in OPNc-CM with or without 1 μl OPN-neutralizing antibody added to 1 ml fresh medium for conditioned culture. e Cell survival from CCK8 assays in H1299 cells subjected to cisplatin doses in OPNc-CM and CM with OPN-neutralizing antibody. f Cell growth in H1299 cells in OPNc-CM and neutralizing antibody. Data were represented as the mean ± SD from 3 (apoptosis) or 6 (viability) independent experiments. * p < 0.05

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