Table 2 Primer properties for the amplification of genes of interest [22]
From: AXL receptor tyrosine kinase: a possible therapeutic target in acute promyelocytic leukemia
Gene/ Primer | Sequence | GC% | Annealing Temperature |
|---|---|---|---|
GAPDH-Forward | CCTGCACCACCACTGCTTA | 57.8% | 59.9 °C |
GAPDH-Reverse | CATGAGTCCTTCCACGATACCA | 50.0% | 59.5 °C |
AXL-Forward | GTCGGACCACTGAAGCTACC | 60.0% | 60.1 °C |
AXL-Reverse | CATCGTCTTCACAGCCACCT | 55.0% | 60 °C |
c-myc-Forward | CAGCGACTCTGAGGAGGAAC | 60.0% | 59.8 °C |
c-myc-Reverse | TCGGTTGTTGCTGATCTGTC | 50.0% | 58.2 °C |
AXIN2-Forward | TCAAGTGCAAACTTTCGCCAACC | 47.8% | 62.8 °C |
AXIN2-Reverse | TAGCCAGAACCTATGTGATAAGG | 43.4% | 57.0 °C |
HIF1α-Forward | CAGATCTCGGCGAAGTAAAG | 50.0% | 60.4 °C |
HIF1α-Reverse | TCACAGAGGCCTTATCAAGATG | 45.5% | 60.8 °C |