Fig. 4
From: AXL receptor tyrosine kinase: a possible therapeutic target in acute promyelocytic leukemia
Effect of R428 on ATRA resistant NB4 cells. Cells were cultured in RPMI medium + 10% FBS + 1% L-Glutamate and 1% Penicillin and Streptomycin in the presence of DMSO, 1 μM, 2μΜ and 3 μM of ATRA for first, second and third months respectively (A) Methodology for development of ATRA resistant NB4 cell line. (B) MTT assay performed for the first, second and third month for development of ATRA resistant NB4 cell line in the presence of 0.01% DMSO and indicated concentrations of ATRA. (C) q-PCR for the expression of AXL-RTK in ATRA sensitive and resistant NB4 cells. (D) MTT assay for R-NB4 cells treated with indicated concentration of ATRA. (E) MTT assay for R-NB4 cells treated with indicated concentration of R428 (p-values < 0.05 are statistically significant). Bars show mean +/− SD