Table 1 Primers used in this study
From: A positive-feedback loop between HBx and ALKBH5 promotes hepatocellular carcinogenesis
Application and amp icon | Sequence (5′ → 3′) |
|---|---|
Oligos for RT-qPCR | |
GAPDH-qpcr-F | AACGGATTTGGTCGTATTGGGC |
GAPDH-qpcr-R | TTCGCTCCTGGAAGATGGTGAT |
HBx-qpcr-F | CCGTCTGTGCCTTCTCATCTGC |
HBx-qpcr-R | ACCAATTTATGCCTACAGCCTCC |
ALKBH5-qpcr-F | GGCCTCAGGACATCAAGGAGC |
ALKBH5-qpcr-R | ACAGGGACCCTGCTCTGAAAC |
siRNA for knockdown | |
siWDR5a1 | CACCUGUGAAGCCAAACUU |
siHBxb2 (duplex mixture) | GCAUACUUCAAAGACUGUUUG GGUUAAAGGUCUUUGUACUAG GGCAUAAAUUGGUCUGUUCAC GUUUUUCCCCUCUGCCUAA |
Oligos for RT-qPCR | |
ALKBH5-TSS-F1 | AAATATTCGGACGATGCCGTGACGCGG |
ALKBH5-TSS-R1 | TTTATACGGGCATGCGCGTGCGTGCA |
ALKBH5-TSS-F2 | TATAGGACCCTAGAGCAGCGTCGT |
ALKBH5-TSS-R2 | AAATATGTGTCCGGGGCCAAGCG |
Oligos for MeRIP-qPCR | |
HBx-3’UTR-F | CCGTCATCTCTTGTTCATGTCC |
HBx-3’UTR -R | CGGCCGCTCCAAATTCTTTATAAGGG |
Oligos for plasmids construction | |
EcoRI-HBx-F | GGTGAATTCATGGCTGCTAGGCTGTGCTGC |
NotI-HBx 3UTR WT-R | CTTAGCGGCCGCTCCAAATTCTTTATAAGGGTCG |
NotI-HBx 3UTR A1907C-R2 | TTAGCGGCCGCTCCAAATTCTTTATAAGGGTCGATGGCCATGCCCCAAAGCCACCC |