Fig. 5
From: A positive-feedback loop between HBx and ALKBH5 promotes hepatocellular carcinogenesis
ALKBH5 promotes the growth and migration of hepatoma cells. A Efficiency of ALKBH5 knockdown (KD) in HepG2 and HepG2.2.15 cells were evaluated by Western blot assay. B Proliferation rates of HepG2 and HepG2.2.15 with or without silencing of ALKBH5 were detected by CCK8 assay. C Effect of knockdown of ALKBH5 on migration rates of HepG2 and HepG2.2.15 were detected by transwell assay. D, E Tumor size and weight in each group. 1 × 107 of HepG2.2.15 cells (control) or HepG2.2.15 cells with ALKBH5-knockdown (KD) were subcutaneously injected into nude mice, and the tumor size (D) and weight (E) were measured 3 weeks after tumor cells inoculation