Fig. 2

Co-culture with hepatocytes resulted in the re-expression of E-cadherin in CRC cells. (A) PRL-3 expression in stably transfected cell lines (LoVo-P and LoVo-C) was analyzed by western blotting. GAPDH served as the loading control. (B) LoVo-P cells were co-cultured with human hepatic LO2 cells for 0–3 days. The protein expression of E-cad and Snail were analyzed by western blotting. GAPDH served as the loading control. **P < 0.01. The immunoreactivity of E-cad (C) and Snail (D) detected by immunofluorescence staining. The nuclei were visualized with DAPI staining (blue). E-cadherin and Snail were immunostained with red, and PRL-3 was immunostained with green, and merged with blue. Original magnification, 400×. (E) Src and p-EGFR protein expression in LoVo-P cells co-cultured with LO2 cells was evaluated by western blot. (F) Images of invaded cells. Cells were co-cultured with human hepatic LO2 cells for 0/3 days and then harvested to perform invasion assays. The data represent the average of three independent experiments. **P < 0.01