Fig. 4

HOTAIR enhanced the degree of H3K27me3 binding to the PPARα promoter. A EZH2 and H3K27me3 proteins bind to the promoter region of the PPARα gene in different grades of glioma. B ChIP analysis of the H3K27me3 protein in the promoter region of the PPARα gene in different treatment groups in the U87 cell line. C ChIP analysis of the H3K27me3 protein in the promoter region of the PPARα gene in different treatment groups in the U251 cell line. D Quantitative PCR analysis. E Western blot analysis; full-length blots are presented in Supplementary Fig. S1. F) Immunofluorescence analysis. Bar, 20 μm. The data are shown as the mean ± SD. *, P < 0.05; **, P < 0.01