Table 1 Primers and probes used in MSP and QMSP
From: SOX14 hypermethylation as a tumour biomarker in cervical cancer
|  | Primers or Probes | Sequence(5′ to 3′) | Size (bp) |
|---|---|---|---|
MSP | SOX14-methylation primer | F:GTTCGTGGGGGTTTTCGAC | 85 |
R:CAAAAAATAAAACGCCGAAACCG | |||
SOX14-unmethylation Primer | F:GTTTGTTTGTGGGGGTTTTTGATG | 94 | |
R:TCCAACAAAAAATAAAACACCAAAACCA | |||
QMSP | SOX14 primer | F: GTTCGTGGGGGTTTTCGAC | 85 |
R: CAAAAAATAAAACGCCGAAACCG | |||
SOX14 probe | 6-FAM-TGAGCGCGTTCGAGAAAGTTCGGG-BHQ1 | ||
ACTB primer | F: TGGTGATGGAGGAGGTTTAGTAAGT | 100 | |
R: AACCAATAAAACCTACTCCTCCCTTAA | |||
ACTB probe | 6-FAM-ACCACCACCCAACACACAATAACAAACACA-BHQ1 |