Fig. 6

Evaluation of apoptosis and necrosis induction of compound 9 in melanoma cells. A Analysis of B16F10 cells by flow cytometry after treatment with compound 9 with the concentrations of 2.6, 5.3 and 10.6 μM for 24 h. Viable cells (Annexin V−/IP-), early apoptosis (Annexin V+/PI-), late apoptosis (Annexin V+/PI+) and necrosis (Annexin V−/PI+). The negative control was treated with vehicle of the tested compound (DMSO 0.05%). Doxorubicin (10 μM) was used as positive control. B Graphs of percentage of live, apoptosis and necrosis cell. Data presented with mean ± SD values from three independent experiments. The analysis between groups and negative control was performed with ANOVA, followed by Dunnett post-test. (*) p < 0.05, (**) p < 0.01 and (***) p < 0.001