Fig. 2

Confirmation of NDV persistently infected EJ28P cells. (A) Microscopic image and (B) neutral red staining of mock- and NDV-infected EJ28P and EJ28 cells (MOI of 1) at 120 hpi. (C) Virus titre of the culture supernatant collected from EJ28P and NDV-infected EJ28 (positive control) cells. (D) Relative viability of mock- and NDV-infected EJ28P and EJ28 cells (MOI = 1) from 0 hpi to120 hpi. (E) Apoptosis analysis of NDV-infected and mock-infected EJ28 and EJ28P cells stained with Annexin V and propidium iodide, followed by flow cytometric analysis. Percentage of early and late apoptotic cells are presented in a bar graph. All data were presented as mean of biological triplicate readings. Value with ****, ***, **, and NS indicate a P value of < 0.0001, < 0.001, < 0.01, and not significant, respectively