Fig. 4

MCM6 knockdown inhibited the growth of neuroblastoma in vivo. (A-B) BLAB/c nude mice were sacrificed 45 days after subcutaneous injection and the tumor was dissected. (D) The wet weight of tumors formed by MCM6 knockdown cells were significantly smaller than that of the control (p < 0.05). (E) The knockdown efficiency of the lentivirus tool on MCM6 in SK-N-BE [2] cells reached more than 75% (p < 0.0001). (F) H&E staining of subcutaneous tumors in BALB/c nude mice (× 400). (G) Immunohistochemical staining of subcutaneous tumors in BALB/c nude mice on target of MCM6, CDK4, CyclinD1 and Ki-67 separately (× 200). The value is displayed as the mean ± SEM and statistical significance is expressed as *p < 0.05, **** p < 0.0001